FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245945
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31540.25314119002042623No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29650.23797198110671017No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.15169208913716095No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.13034283214748643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20000.044.1993142
GTGGTAT19850.043.2695351
GTATCAA68100.038.773571
TATCAAC76300.034.7569962
GGTATCA37250.033.559261
AACGCAG82150.032.178446
TCAACGC82700.032.174
ATCAACG83200.031.9766643
CAACGCA84750.031.3416925
ACGCAGA92400.028.5628687
CGCAGAG94400.027.9577228
GTCGAGG10450.026.8416868
GCAGAGT100150.026.2697989
AAGTACG1851.0513759E-925.2820511
CAGAGTA58200.023.1136110-11
TCGAGGT12000.022.6681339
AGAGTAC56750.019.51007310-11
AGTCGAG16800.019.4788637
TACGGGT14650.019.4365584
AGTACGG71650.019.277442
Produced by FastQC (version 0.11.5)