FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245945
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17840.1431844904871403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24600.037.5590821
TGGTATC24550.037.5100142
GTATCAA77950.023.5652031
GGTATCA44200.021.6504941
AGAGTAC58100.021.1589711
TATCAAC86750.020.9769522
CAGAGTA59250.020.8875410
AACGCAG92950.019.579316
ATCAACG94100.019.3369263
TCAACGC93850.019.3298594
CAACGCA95150.019.1235395
TACCGTC750.007422968318.328737
ACGCAGA105300.017.4671237
CGCAGAG106750.017.1546678
TAATACG1152.6300317E-416.7308964
GGTACGG1152.6300317E-416.7308963
GCAGAGT111250.016.5850169
AGTACGG73350.016.57239513
CGGTAGA1354.8067515E-516.2954751
GAGTACG74800.016.28790712
Produced by FastQC (version 0.11.5)