FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776744
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28240.15894242502014921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23000.034.0868531
TGGTATC23500.033.5739672
GTATCAA75650.026.9450631
TATCAAC84250.023.8526212
AGAGTAC63050.022.89927911
CAGAGTA63750.022.73347310
ATCAACG88800.022.5040763
TCAACGC89100.022.3974534
AACGCAG91000.022.1122976
CAACGCA91400.021.9842135
GACCGGT650.003237677321.15695438
CGATCGT650.003237677321.15695447
GGTATCA40200.021.144741
ACGCAGA100100.020.1289847
CGCAGAG102100.019.7116458
GCAGAGT108550.018.670719
AGTACGG77350.018.48854813
CGGGTCG750.007410766618.33447817
CGCGACC909.814411E-418.33447812
CCGACCG750.007424017418.328831
Produced by FastQC (version 0.11.5)