FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033219
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.24873719898685565No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.23121913166521327No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.1542751343132482No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13760.13317602560541375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16800.048.062252
GTGGTAT17550.045.7883151
GTATCAA54750.036.7322041
TATCAAC62100.033.19022
TCAACGC64900.031.6927854
ATCAACG65300.031.5637283
CAACGCA66000.031.1001825
AACGCAG66300.031.0235586
GGTATCA30650.030.795871
GTACGGA1353.517198E-828.3451461
GGTACGG600.006807503828.331433
ACGCAGA73700.027.9099257
CGCAGAG74300.027.7430848
GCAGAGT79600.025.897129
CAGAGTA46950.023.49224910-11
GTGTAGG3500.023.0810471
GCTTGTA5050.022.7322461
GTCGAGG8700.022.471938
ACCGATC950.002342544722.3690838
ACGGGTA5150.022.2800565
Produced by FastQC (version 0.11.5)