FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033219
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17280.16724431122540334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17050.036.752032
GTGGTAT18100.034.943981
GTATCAA61000.025.5609111
AGAGTAC47600.023.57661811
CTAGTCC952.3277735E-623.1439363
CAGAGTA49900.022.87466810
TATCAAC68900.022.696762
GGTATCA32750.022.5872881
ATCGGTC1558.9858077E-1021.2950114
ATCAACG74250.020.9503273
TCAACGC74200.020.9274064
AACGCAG75100.020.6786146
TACGTCA804.4599624E-420.6125684
CAACGCA75950.020.5175785
GCTTGTA3350.019.7010571
ACGCAGA83550.018.6201367
CGCAGAG83550.018.5588328
AGTACGG60050.018.36790713
CGGGTCG1503.1551644E-718.33648117
CCTAGTC909.854563E-418.3222832
Produced by FastQC (version 0.11.5)