FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344742
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41690.3100222942393411No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.24131022902534466No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.17698562252089992No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.1279055759394739No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.11667665619129915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16100.048.853891
TGGTATC16800.046.799162
GTATCAA63700.043.3837621
TATCAAC73550.037.9628032
ATCAACG77600.035.8172043
TCAACGC77750.035.638784
GGTATCA32850.034.944731
CAACGCA79450.034.9297035
AACGCAG81400.034.0407266
ACGACGC703.9443004E-430.3562158
ACGCAGA91500.030.2832267
CGCAGAG93700.029.5268428
GCAGAGT98500.028.1311199
GTCGAGG12000.027.9783128
GACAGGT8750.027.6961961
TCGAGGT11550.025.7567869
AGTCGAG16450.025.5767237
CAGAGTA52750.024.97646510-11
CGTCAGA2501.8189894E-1223.799275
AGAGTAC50700.022.59137210-11
Produced by FastQC (version 0.11.5)