FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344742
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26720.19869982494783384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22300.041.6531262
GTGGTAT22600.040.8722081
GTATCAA82200.026.6553131
AGAGTAC55050.024.18217511
TATCAAC91250.023.8822922
CAGAGTA56700.023.86653510
GGTATCA42150.023.0142443
GTACGAC600.00203367522.92060511
TCAACGC95750.022.5007444
ATCAACG96300.022.4863783
CAACGCA97300.022.1423035
AACGCAG98650.021.8982626
AGTACGG63450.020.85070813
GAGTACG66100.020.05639612
ACGGGGC5250.019.90818416
TACGGGG27150.019.85608315
ACGCAGA110000.019.6644787
GGGCGTA700.004986521319.63600231
CGCAGAG111550.019.3694788
ACGGGAT13050.018.96877716
Produced by FastQC (version 0.11.5)