FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326141
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.3630331666365161No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.3121349355033559No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.2060458513342389No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.172624723662465No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.1404300593914902No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.10394277321771872No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG200.003209980263.807211
GTGGTAT4450.054.4870531
TGGTATC4700.051.5413442
GTATCAA19150.045.314781
AATTAGG505.444108E-542.4992
TATCAAC21250.041.5990262
ATCAACG22850.038.1282123
TCAACGC23200.037.5529984
GGTATCA9000.036.8663831
CAACGCA23700.036.581425
AACGCAG24650.035.1715936
GTCGAGG3300.033.4840628
TCGAGGT3150.032.3801969
CGCAGAG27300.031.7574988
ACGCAGA27600.031.2583267
TCCTATA550.004443894630.9083632
GCAGAGT29200.029.6910849
AGTCGAG4650.029.2466287
CGGTTGC300.00680423828.33266884-85
CACACCG600.006799323528.3326686
Produced by FastQC (version 0.11.5)