FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655734
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68980.4166128134108498No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.310194753505092No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34100.20595095589025772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18150.056.9179761
TGGTATC19100.054.2980732
GTATCAA69100.039.7442471
GGTATCA37550.036.1160741
TATCAAC76600.035.900752
TCAACGC82000.033.3780254
ATCAACG82150.033.3688133
CAACGCA83850.032.6922845
AACGCAG86700.031.8137026
ACGCAGA95400.028.9107067
CGCAGAG95600.028.8057738
GCAGAGT99850.027.6222489
CAGAGTA57550.024.92245710-11
GTATAAC1754.335452E-721.863731
GAGTACG65250.021.78536612-13
AGAGTAC54950.020.99735310-11
GTCTACG1455.4945776E-520.5234261
GTACGGG68800.020.35242814-15
ACGGGAT9750.018.95980816-17
AGTACGG61300.018.64837812-13
Produced by FastQC (version 0.11.5)