FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666927
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13580.20362048620013884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC23850.038.2556042
GTGGTAT24000.037.9162181
CGGTATC558.737734E-734.9768522
ATCGGTA606.306803E-527.50450528
GACGTCA507.0313195E-427.49831244
CTTAGAC606.345596E-527.4797533
TGAGTAC550.001231289824.9815944
GTATTAG2000.024.742911
GTATCAA80750.024.6492821
AGCTCCG701.7956925E-423.57882752
AGAGTAC49650.022.93426711
CAGAGTA50450.022.73414810
GGTATCA43050.022.7242553
TCGGTAT852.5679561E-522.64736629
GCGGTAT852.5739197E-522.6405721
TATCAAC89300.022.2501132
GTACATA1155.0503695E-721.5155741
TCTACGC903.9827326E-521.39560347
GACAGGT2350.021.0577961
CGGGGTA1207.570525E-720.62837817
Produced by FastQC (version 0.11.5)