FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211357
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA18410.15197831853037544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17290.14273248926617008No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13460.11111505526446787No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT13030.10756531724338902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC30450.030.1461582
GTGGTAT32350.028.724911
CGGTCGG507.0261816E-427.50398634
GTATCAA103850.026.2351421
AGAGTAC77650.024.93497511
CAGAGTA79350.024.60872710
TATCAAC115250.023.5368862
AACGCAG115900.023.3100326
CGCGGCC1553.6379788E-1123.06690611
ACGCTAG600.002033388522.92093524
GACAGGT3400.022.6409871
TCAACGC122900.021.935834
ATCAACG123500.021.9182663
CAACGCA124750.021.6536925
CCCGCGG1801.0913936E-1121.39552552
GCTTGTA5900.020.9689541
CGCGGAT804.4331737E-420.631449
TGCCGAC804.4443103E-420.62372855
ACGCAGA132500.020.412957
GCCGAGC956.0531835E-520.26609622
Produced by FastQC (version 0.11.5)