FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944055
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40140.2064756398352927No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37460.19269002163004648No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.1307576174542387No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.11203386735457586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25850.046.0321852
GTGGTAT26900.044.4116481
GTATCAA91600.036.7597471
GGTATCA51200.034.543491
TATCAAC101750.033.204492
TCAACGC105800.031.7727574
ATCAACG107850.031.208233
AACGCAG107600.031.162256
CAACGCA108200.030.9501695
ACGCAGA119450.028.0003747
CGCAGAG121100.027.6202898
GCAGAGT131850.025.4657069
GTCGAGG13500.024.2412268
CAGAGTA79450.023.11052510-11
TCGAGGT14600.021.250979
ACGGGTC6850.019.8528793
AGAGTAC77700.019.58312210-11
GAGTACG97700.018.70655412-13
TACGGGT17900.018.5184944
AGCGTCA4600.018.4772033
Produced by FastQC (version 0.11.5)