FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944055
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31970.16445007985885174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29150.035.653342
GTGGTAT29650.035.43671
GTATCAA104900.026.2990471
TATCAAC116400.023.5499042
AGAGTAC80350.023.170711
CAGAGTA81550.022.89718810
GGTATCA55250.022.3028471
ATCAACG124750.022.015433
TCAACGC124550.022.0066364
AACGCAG126000.021.7970226
CAACGCA127600.021.5446975
CGATCGT1351.0419535E-720.37368847
ACGCAGA141600.019.3593167
CGCAGAG143200.019.0868828
AGTACGG101450.018.21649213
GAGTACG102700.018.1554412
GCAGAGT151500.018.1324169
GTCCGGA1454.6427485E-617.06910955
GCTTGTA6550.016.3771341
CCGGTCG1601.1835224E-515.4676869
Produced by FastQC (version 0.11.5)