FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683113
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.22618802183810593No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35680.21198814339857158No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22600.13427500114371405No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT20150.11971864040025834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23700.046.4670371
TGGTATC24300.045.8228532
GTATCAA80450.039.8509671
TATCAAC90100.036.0375862
TCAACGC94950.034.19684
ATCAACG96450.033.7530983
AACGCAG96950.033.7095346
CAACGCA97050.033.5444225
GGTATCA43550.031.7313671
GTCGAGG10900.030.0219178
ACGCAGA110850.029.4441997
CGCAGAG110950.029.417668
GCAGAGT121750.026.8446279
CAGAGTA72550.024.1071210-11
TCGAGGT13100.023.3593739
GCTTGTA7600.022.3789631
AGAGTAC70050.021.3876910-11
AGCGTCA4600.021.2499123
AGTCGAG19850.020.7675577
GAGTACG91250.019.09751312-13
Produced by FastQC (version 0.11.5)