FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683113
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27370.16261534430546254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25200.038.1695822
GTGGTAT25900.037.468541
GTATCAA92750.026.8538231
TATCAAC101350.024.4586582
AGAGTAC71850.024.26950511
CAGAGTA74150.023.7756510
TACCGTC701.8072533E-423.5595847
ATCAACG108850.022.7222353
TCAACGC109400.022.6324464
AACGCAG109950.022.4969146
CAACGCA111050.022.2219335
GGTATCA46400.021.7954983
CGCATAT650.003237523621.1570512
CGCGTAG804.4389183E-420.62812234
ACGCAGA124850.019.8138287
CGCAGAG125650.019.7349438
CACGCGT856.698939E-419.40604632
ATACGCA856.698939E-419.40604632
AGTACGG89700.019.19465613
GCTTGTA4500.018.9383011
Produced by FastQC (version 0.11.5)