FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425006
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40030.2809110979181842No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.2736830581765971No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24990.17536768266238878No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.15354321315138322No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15880.11143812727806059No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15460.108490771266928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC27150.043.203212
GTGGTAT26900.043.1413541
GTATCAA83650.037.198771
TATCAAC94600.033.244312
TCAACGC100850.031.0997714
GGTATCA47600.031.078191
AACGCAG100850.030.931216
ATCAACG101550.030.8853993
CAACGCA102700.030.498175
ACGCAGA112400.027.7527797
CGCAGAG113500.027.5596648
GCAGAGT121600.025.7588169
GTCGAGG12000.024.0835048
CAGAGTA68750.023.24461610-11
GTACGGA2104.5729394E-922.2667481
TACGGGG37700.021.3057692
TCGAGGT14500.019.9311739
ACGGGTA5350.019.8592555
AGAGTAC66800.019.81932310-11
CTCCGGT2151.4325633E-719.766897
Produced by FastQC (version 0.11.5)