FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427746
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.1884789031102171No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.17643194237630502No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.12411171174704745No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA14470.10134855919750431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19400.047.1167641
TGGTATC19400.046.441422
GTATCAA65900.037.4180641
GGTATCA37650.034.1020661
TATCAAC74900.033.0794372
TCAACGC78200.031.5748124
ATCAACG78800.031.3883273
CAACGCA79950.030.9899925
AACGCAG80800.030.6113876
ACGCAGA89450.027.6987137
CGCAGAG90550.027.4091638
GCAGAGT98450.025.2106329
GTCGAGG8500.022.499098
CAGAGTA63750.022.03321610-11
GCTTGTA5450.020.2822251
AGAGTAC61400.019.27717610-11
TTAGACG1358.0351275E-418.8881264
GACAGGT7050.018.0913451
GAGTACG78150.017.9189712-13
TCGAGGT10300.017.7426229
Produced by FastQC (version 0.11.5)