FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427746
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21500.1505870091739007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22250.038.2982031
TGGTATC22700.037.4060972
CGATCGT957.948256E-826.06156747
GTATCAA78050.025.709681
CCGATCG1052.0937296E-723.57786246
AGAGTAC63300.022.81188211
TATCAAC87850.022.5842292
CAGAGTA65150.022.4174210
ATCAACG90800.021.7891963
GGTATCA41850.021.7409881
TCAACGC91050.021.669014
CAACGCA91750.021.5036875
AACGCAG92400.021.3821546
GTCCGGA1251.1234315E-619.80054355
ACGCAGA101350.019.4417637
CGCAGAG102150.019.2969388
GCTTGTA3500.018.849071
AGTACGG76550.018.57594113
GATCGTA1352.3397843E-618.33962248
GCGATAG1503.159712E-718.33512121
Produced by FastQC (version 0.11.5)