FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061386
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65620.6182482150697296No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7950.035.6518481
TGGTATC8000.035.4006352
GTATCAA39400.030.1018371
TATCAAC44150.027.2776682
GGTATCA18100.026.6055011
TCAACGC45300.025.9065344
CAACGCA45650.025.8295365
ATCAACG45900.025.6338023
AGAGTAC42800.025.24954611
AACGCAG47350.025.1947926
CAGAGTA44350.024.61162210
ACGCAGA48900.024.283767
CGCAGAG49250.024.0553728
GCAGAGT52000.022.840389
AGTACGG48750.021.71962513
GAGTACG49650.021.43366812
CCGATCG700.00496814419.64807739
ACGGGTC1552.0827429E-819.51855516
GTCGTAC856.65457E-419.4242521
GTACGGG53550.019.36281814
Produced by FastQC (version 0.11.5)