FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634337
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.24907895960664442No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.22022994086739384No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.14645212245226116No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.1303723415156297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9750.046.20472
GTGGTAT10300.043.3350871
GTATCAA35650.039.5882381
TATCAAC39400.035.9197162
ATCAACG42400.033.2779923
TCAACGC42950.033.1487054
AACGCAG43250.032.8205036
TACGTAT652.5522095E-432.6920055
CAACGCA43950.032.2977685
GGTATCA19000.031.3229061
CGCAGAG48050.029.541878
ACGCAGA48150.029.4805167
GCTTGTA3850.028.707821
GCAGAGT51850.027.3767959
AAGTACG1302.3648685E-522.8898141
CAGAGTA32100.022.17658610-11
GTCGAGG5150.021.456118
GCAGCGT1404.190264E-521.254831
ACGGGAG5200.021.2498025
GACAGGT2651.4006218E-1020.8537941
Produced by FastQC (version 0.11.5)