FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634337
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8830.13920045653966268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13650.037.858032
GTGGTAT14500.036.414261
ACTATCG1103.929017E-1027.50686511
GCTTGTA1700.025.8826871
GTATCAA44950.025.267291
CGGCATT550.001224728325.00426728
ACCGTTA550.001225291625.00229635
AGAGTAC33950.023.901411
CAGAGTA35000.023.34153710
ATCGGAT600.002032511222.92057825
CGAACTA600.002034376622.91696229
TATCAAC50500.022.5886122
GGTATCA24300.021.8297923
ACGGACG650.003248306521.1424473
TCAACGC54400.021.1191254
ATCAACG54400.021.0180783
AACGCAG54450.020.9987776
GACGTTA804.4554274E-420.6138846
CAACGCA55750.020.6077215
TCGGTCA1351.0382246E-720.37545615
Produced by FastQC (version 0.11.5)