FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518168
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA9450.182373284340214No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT7010.13528430933596827No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.13026663167158142No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC6730.12988065646662858No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA6480.12505596640471817No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT6180.11926633833042564No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC5440.10498525574717081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20000.044.6324461
GGTATCA10300.041.6817131
TATCAAC24150.037.666742
ATCAACG25000.036.379053
TCAACGC25100.036.2341164
GTGGTAT4700.036.176251
TGGTATC4850.035.9338342
CAACGCA25900.035.443095
GTCTACG601.592758E-435.422581
AACGCAG26050.035.4021456
CGCAGAG27500.033.690038
ACGCAGA27800.033.3264667
GCAGAGT29700.031.1944719
GTATTAT1054.512267E-628.3380621
GTCACGA300.0068029428.33532772-73
CAGAGTA23300.026.3566110-11
ATACCGC1001.05501196E-425.4993326
ATTAGAG900.001710061923.6104953
GAGTACG27500.022.25396512-13
GACAGGT2305.3842086E-1022.1776141
Produced by FastQC (version 0.11.5)