FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518168
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT7560.1458986274721712No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT6490.12524895400719457No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT5840.11270475984622746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5690.10980994580908122No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT5640.10884500779669914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC501.8219876E-532.989633
GTATCAA22350.031.1470321
GTGGTAT4850.028.3662471
TGGTATC4950.027.7770962
CCGTTAT400.00784792927.5152631
CGAATCA400.00786644427.50197833
TACTAGA507.027681E-427.4993232
TATCAAC25400.027.174532
GGTATCA10000.026.9649561
TCAACGC26400.026.345894
AACGCAG26650.026.3050546
ATCAACG26550.026.3005893
CAACGCA26750.026.1039495
AGAGTAC26900.025.76144811
CGCAGAG27500.025.4944528
CAGAGTA27500.025.29937710
ACGCAGA27800.025.2193327
CCGAAAT550.001225130725.00179947
GCAGAGT29400.023.8514549
AGTACGG29350.023.14253213
Produced by FastQC (version 0.11.5)