FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length91
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAATGCTGCAAAGCGTGCCTGAAAACCAAATAGCAATGCCAGACCAC11.1904761904761905No Hit
GTTTTACTCTAGATTTCACTGTCGTCCCACACCACTTCTTCCACCCCACT11.1904761904761905No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATT11.1904761904761905No Hit
AGAGTGGAGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAGT11.1904761904761905No Hit
AAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAACCGAACGAGGGG11.1904761904761905No Hit
GAGCCGATGAAGGACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGTCAA11.1904761904761905No Hit
GCATGCCTACTGCCTTTCACCTGGACAACCAACGCCAGGCTGACTTAACT11.1904761904761905No Hit
GATTTTCATATCTACGTTGAGCCTGAATCGCCTGAACCCGTTGTTCATCC11.1904761904761905No Hit
AAATAATAACACAATACCTTAAAATGTTCCACTCACAATTATTCGAAGTG11.1904761904761905No Hit
AATCAGTATTTAGCTGACCAATGAAAACACATTAATAGAATTTTAAAAAC11.1904761904761905No Hit
GAATGAGTCAGGTAAAAAAAAAAAATAGGCACATTAAGGAAGAAGGATTA11.1904761904761905No Hit
AATAAAAACTCAGATAAAATAATATATTAATTAAAAAACGAAATTTTAAG11.1904761904761905No Hit
AGGCAGGCGCGGCCTTCGTTGTCAGTAGTTCTTTGATGTGAAAGGGGCAG11.1904761904761905No Hit
GACAGGCTGGGGACCGCGGGGCGGACGCGAGCGAGTATCTCCGCTCTGAC11.1904761904761905No Hit
GTATTGGTTGTTGGACTTATTCAAAAAGTTCGTGGTAAATCAACAACAGC11.1904761904761905No Hit
GGTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGC11.1904761904761905No Hit
ATAAACTCATAATACAAATTAATTTTAATAATATTTTTTTTTATTAAAAT11.1904761904761905No Hit
GTATCAACGCAGAGTACGGGCAATTATCCTCGGCTAAGGATAGTTCGGAA11.1904761904761905No Hit
CATAACAAACAATCAACTAAGATAAAAATATTATAATATAAAAAAAAATA11.1904761904761905No Hit
GTATCAACGCAGAGTAGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT11.1904761904761905No Hit
ATCAACGCACAGTACGGGCAAATAGAAAAAATAAATAAGAATATGTGTTG11.1904761904761905No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11.1904761904761905No Hit
GTCATATATTTTCGGCACAATAATATGACTACCTTGAATTAGACGAATTC11.1904761904761905No Hit
CATACAGGGACACTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG11.1904761904761905No Hit
GGCAAATACAACTTAAAAACACACTAATGAAGAAAAATAAAATGAATAAA11.1904761904761905No Hit
ATCACCGTCAGCAGCGTACCTTCTCCCGAAGTTACGGTACCATTTTGCCT11.1904761904761905No Hit
AAAAGAAAACAATCGTGGACACAGAAAAAACAAAGCAACACGCAGGCCTC11.1904761904761905No Hit
AAAAAAACAAAAAAAATAAAATAAAAAAAAAAAAAAATAAAAAAAAAAAA11.1904761904761905No Hit
CTCAAAAAATTAAGTTCATAATCAGCATGAATATGAACTAAGATAAAATC11.1904761904761905No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA11.1904761904761905No Hit
GTACGGCCATCGGCGGTGATGTTAAATAAAACTTCTGATAAATCAGGCAC11.1904761904761905No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1904761904761905No Hit
GTATCAACGCAGAGTACGGGATTGTGAACTTTGGGGGAAGCCCCTCCAAC11.1904761904761905No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTAGATGGAGCAA11.1904761904761905No Hit
CTTCAGGAACATCCAAACCTTCAAAATGATCTGGCTTGGTATATTGTGGG11.1904761904761905No Hit
TTCCAAAACTAAGTACTGATAAACTAATAAACAAATAAAATCCAACAGTA11.1904761904761905No Hit
ATGGTAGAAACGAAGACTACTTAAAAATTTTTATGGGAAATTAACTATAA11.1904761904761905No Hit
TCGCGCAAAGTGATCAAATCGATTACAACCTGAAAGAAAACTGGTCAGTT11.1904761904761905No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT11.1904761904761905No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11.1904761904761905No Hit
CCCATCGACTACGCCTCTCGGCCTCGCCTTAGGGGTCGACTCACCCAGCC11.1904761904761905No Hit
GAACATCCTTCCTTAGTTTCCTTTGCCTTCAATTCATTAATCTCTTCTGT11.1904761904761905No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1904761904761905No Hit
ACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACT11.1904761904761905No Hit
GCGATTCTGTAAAGATATCTTTGTCCCTGAGGTGTCAGTTTGCGGCCGCC11.1904761904761905No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1904761904761905No Hit
GGCGGCTTTTTAAGTCGGATGTGAAATCCCCGAGCTTAACTTGGGACTGT11.1904761904761905No Hit
CGCAAGTTGTGGATCATTGATACCAAGTGCACTTAACACTTCGTCACAAG11.1904761904761905No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCTGCATATGGTTTTGGGT11.1904761904761905No Hit
GTGCCAGGTAACGCCTGGGCGGTGTGAACCGACGGAAAGTGCAGCAGAGA11.1904761904761905No Hit
GTATTTATTATTAGTAAGAGAATTTAAATCGTTTTAAACCATAAAACCTA11.1904761904761905No Hit
GACAGGTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACTGAA11.1904761904761905No Hit
CATCCGCCTTCGCTCGCCACTACTAACGGAATCACGGTTGTTTTCTCTTC11.1904761904761905No Hit
ACCTAACCTACAGACATCCACACATGAGAACCAAAAAAAAAAATAAAAGA11.1904761904761905No Hit
GCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGA11.1904761904761905No Hit
ATCCTCTTTCTATCCTTCTTTTTTTCTCGCTATTATTTTCTTGTGCTTTT11.1904761904761905No Hit
GTATCAACGCAGAGTACGGGGATCGACACAGGGAATGAGAACGGAAAATA11.1904761904761905No Hit
CCAACTCGGTGGCCTAGAAGTCATGCACTGGCTATCAGATGCACGCGATA11.1904761904761905No Hit
ATCTTATGCCTCACCGCAAAAAAAAAAAAACACAAAAACCAAACCTGTCT11.1904761904761905No Hit
CGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACAT11.1904761904761905No Hit
ACAAACATACAAGTTTAATTTAAAAAAATTAATATTATTTGATACCACAC11.1904761904761905No Hit
CTTAGATGCTTTCAGCGGTTATCCCTTCCGAACATAGCTACCCGGCGATG11.1904761904761905No Hit
ATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGT11.1904761904761905No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT11.1904761904761905No Hit
AAGTACGGGATTATTATACAACCCTGTCCAAAATGAAAAAACCTTGTCTC11.1904761904761905No Hit
TCATATATCTGGCGGACTGGGCAACTTCAGCTTCTGAGAGTTCTGCCCAC11.1904761904761905No Hit
GTGGTATCAACGCAGAGTACGGGAACAAAATTTTAAATATAACAAAAAAA11.1904761904761905No Hit
AAACAGGACTTTGGACAATATGTTACCGCAACAAATTTGACCGTATTAAT11.1904761904761905No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1904761904761905No Hit
CACCTGTCTCGTCCCCAGTGGCTTTTCCAGAACTACTGCCTTCACCATGA11.1904761904761905No Hit
AAACGCAGAGTACGGGCAGTGGTATCAAAGAAAAAAAAAAAAAAAAAAAA11.1904761904761905No Hit
CAGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTTTCA11.1904761904761905No Hit
CAACACATATTATATTTTTTTTTTTTTTTTTTTTTTTTTTATAAGAAAAA11.1904761904761905No Hit
CAGTGACCTCCTTGTGCCAATGGACATAAATCAGGGGATGGCCATGGAAC11.1904761904761905No Hit
AGTATCAACGCAGAGTACGTTAGAAGGAGATATTTATTTATATTAAAAGA11.1904761904761905No Hit
GGGGAAGGAAAAGAAGGAAGAACAGGTCATCAGCCTCGGACCTCAGGTGG11.1904761904761905No Hit
CCTGAGGAGGAGATGGATGAATGTGAGCAGGCGCTGGCTGCTGAGCCCAA11.1904761904761905No Hit
TCATTATGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT11.1904761904761905No Hit
CGACCTATTTAAAAGATTTTTAGTGCAATTAATAATAGGACTAAGGTCAT11.1904761904761905No Hit
ATTTTTATCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1904761904761905No Hit
CACAGGAAGCGATCGGAGGCTCAGAGAACTTCAACAGTGTGTTCGAGGGC11.1904761904761905No Hit
TAACATAATAATAATTAAAATTTAAAAATAAAAAATTATGTAAAAAATAA11.1904761904761905No Hit
TTCCCTCAGTAGCGGCGAGCGAACGGGGATCAGCCCATTAAGTTATATGT11.1904761904761905No Hit
GAATAAGCAGCACTAGTATCAAAGAAAAAAAAAAAAAAAAAAAAAAAAAA11.1904761904761905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGT50.085.04
TGGTAAG50.085.07
ATGGTAA50.085.06
CTTATGG50.085.03
GTAAGTG50.085.09
CCTTATG50.085.02
ACCTTAT50.085.01
TATGGTA50.085.05
GGTAAGT50.085.08
Produced by FastQC (version 0.11.5)