FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005415908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16258993
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC612410.3766592432876993No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA598310.367987119497499No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG477340.29358521773150403No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA334480.20571999754228323No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT255470.1571253521051396No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT252970.15558774150404026TruSeq Adapter, Index 16 (97% over 40bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA209330.12874721085125013No Hit
TGGTGGGGATAGTGATGATTATGGTAGTGGAGGTGAAATATGTTTGTGTG163630.10063968906315415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)