FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005415909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15806472
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT761540.48178999083413426TruSeq Adapter, Index 18 (97% over 40bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC505700.31993224041392665No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT285140.18039446120551125No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA240880.1523932728315338No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG228160.14434593627218018No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA205010.1297000367950546No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT160290.10140782838827032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1031250.0113.322771
CGGGTGC96450.0112.1090851
CGGGCGC268050.0110.666231
CGGGTAC100050.0106.527851
GGGCGCG323650.097.217412
CGGCTAA7350.095.592071
CGGAATA133550.093.315231
CGGGTGT293050.092.9151841
CGGAAAT84350.091.6255951
CGGGTAT324650.091.500891
CGGGTTT820000.091.310641
GGCGCGG338150.090.284283
CGCGGTG333100.089.956765
CGGGCGT416150.089.367441
CGGATGC12800.089.313781
GCGCGGT333800.089.2157444
CGGAGTG90700.088.558831
CGGTAAT106600.088.252521
CGGAATT179450.088.160761
CGGATAA77350.087.2931061
Produced by FastQC (version 0.11.5)