FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005415922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19383358
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC755670.3898550498835135No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT196900.10158198594897748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC355200.0112.728431
CGGGTGC102450.0111.6674041
CGGTTAA751050.0109.955731
CGGGTAC125050.0109.335781
GGGCGCG415900.0102.3008652
CGCGGTG428050.096.376065
GGCGCGG435000.095.1234363
GCGCGGT428050.094.902984
CGGGTAT375900.093.852881
CGGCTAA9050.092.079831
CGGGTGT325100.091.9668351
CGGGCGT502250.090.128951
GGGAGGC467000.090.0368652
CGGAATA151600.089.323811
CGGGAGG1104250.088.77921
CGGGTTT730750.088.427231
GCGGTGG616750.087.90576
CGGTGGT745950.087.712347
CGGTAAT111050.087.153931
CGGGTAA92750.085.931361
Produced by FastQC (version 0.11.5)