FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005415923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11471221
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC515700.44955981582082677No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA276020.24061954695145357No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG155980.13597506316023378No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT151240.13184298341039719No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT143000.12465978992122982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC142700.12439826588643003TruSeq Adapter, Index 4 (100% over 50bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT132350.11537568668583753No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT125760.10963087538806897No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA118590.10338045095635418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC261600.0115.708151
CGGGTGC77650.0113.5335851
CGGTTAA571950.0110.987181
CGGGTAC100750.0110.604171
CGGCTAA4650.0108.811991
GGGCGCG302900.0104.634862
GGCGCGG314850.097.414723
CGGGTAT270050.096.966241
GCGCGGT310300.096.7727054
CGCGGTG314600.096.697975
CGGGTGT248000.095.194481
CGGGCGT358300.094.265611
CGGGTTT549300.090.5956651
CGGTGGT550850.090.1713267
GCGGTGG445150.090.1344766
CGGATAC12750.089.173111
CGGAATA90800.089.093161
CGGGAGG683700.087.84891
GGGAGGC280350.087.584422
CGGGTAA65900.087.528431
Produced by FastQC (version 0.11.5)