FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005415952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17926519
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC570050.317992578481076No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC367810.20517647625844146TruSeq Adapter, Index 6 (100% over 50bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA307930.17177344915652615No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG268320.1496776925849352No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT267630.14929278796402135No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA232460.12967380895309344No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT200350.11176179826100092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC292650.0115.279171
CGGTTAA961550.0113.5638961
CGGGTGC92500.0112.8492051
CGGGTAC114050.0111.813081
CGGCTAA7900.0102.39411
GGGCGCG353550.0102.390512
GGCGCGG369750.094.832863
CGGGCGT398400.094.518331
CGCGGTG370050.094.4467245
CGGGTAT314700.094.19861
GCGCGGT365800.093.8084644
CGGGTGT269800.092.988181
CGGATGC12850.092.57421
CGGAATA169400.091.149531
GCTAATT8500.089.542033
CGGTAAT107000.088.773691
CGGTGGT672800.088.2450947
CGGAATT189950.087.9580461
CGGAGTG87950.087.713661
GCGGTGG542850.087.432186
Produced by FastQC (version 0.11.5)