FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005415965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15558947
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC667540.42903931737796913No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT479860.3084141876696411No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA335010.2153166277897855No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA316610.20349063468112594No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT249910.16062140966223484No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT246350.15833333708251593No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA242180.15565320712256428No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT214300.13773425669487788No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG211520.13594750338824343No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA181310.11653102231147132No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT170100.10932616455342384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC323700.0116.299171
CGGGTGC110750.0115.6930851
CGGTTAA938000.0114.231031
CGGGTAC135700.0113.5783161
CGGCTAA5350.0110.3306351
GGGCGCG383700.0104.878092
CGGGTAT350150.098.625481
CGGGTGT302600.098.537751
GGCGCGG397550.097.78793
CGGAATA216650.096.3492051
GCGCGGT396050.096.233534
CGGACGC13700.096.180371
CGGATGC12050.095.990751
CGGGCGT427800.095.92941
CGCGGTG405550.095.622875
CGGAAAT108850.094.5424961
CGGATAC14650.094.420231
CGGGAGG1004250.094.286761
GGGAGGC433550.093.986032
CGGTAAT117450.092.0871351
Produced by FastQC (version 0.11.5)