FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005415974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19739193
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC847450.42932352908247057No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT380830.19293088628293972No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA314220.15918583905633832No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG291460.14765547912723687No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA290550.1471944673726023No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT252740.1280396822706987No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT218340.11061242473286521No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT214680.10875824558785153No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA199280.10095650820172841No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC140200.0114.6776661
CGGGCGC419950.0113.962511
CGGTTAA1331350.0113.084771
CGGGTAC166450.0109.497631
CGGCTAA7400.0102.9247361
GGGCGCG488600.0102.863342
CGGATGC15800.099.423341
CGGGTGT388800.097.703041
CGGGCGT535300.097.408551
CGGGTAT439750.097.12681
GGCGCGG509300.095.24183
CGGTAAT151300.093.915421
CGCGGTG512600.093.8447345
GCGCGGT508850.093.321034
CGGAATA192200.092.722461
CGGGTTT924150.091.391081
CGGATAC18850.091.227961
CGGAAAT126400.091.0439151
CGGGAGG988200.089.91741
CGGAATT253450.088.955711
Produced by FastQC (version 0.11.5)