FastQCFastQC Report
Wed 11 May 2022
EGAF00005415995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20938125
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC871460.41620727739470464No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA341240.1629754335691472No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT254150.12138145129996118No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT244260.11665801020865048No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT242980.11604668517357691No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT229020.10937942150980567No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT223130.10656637115309991TruSeq Adapter, Index 16 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)