FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005415996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005415996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16630283
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA777560.4675566855957893No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC687470.413384426470674No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA433840.26087349205061633No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG413980.24893142227345139No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT409810.24642394840785328No Hit
TGGTTAGTGGGGGTAGGTTTTTTAGGGAGAGGAGGGTGGATGGAATTAAG333030.20025516102161342No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA299520.18010517319518854No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA284290.17094718111531837No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT274600.16512046126936022No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT254830.15323250963317941No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT225540.13562006130623275No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA198230.11919821208093694No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT182150.10952910422510548No Hit
TGGGTGGAGTGGAATGGAATGTAATGGAGTGGAATGTAATGGAATTTAGT181160.10893380467428004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC324400.0114.871621
CGGGTGC109000.0114.267941
CGGTTAA743150.0113.152141
CGGGTAC125650.0112.684331
CGGCTAA7900.0108.57561
GGGCGCG381850.0101.69152
CGGGTAT326450.098.1845551
CGGGTGT295850.097.306551
CGGGCGT415000.095.879371
CGGGAGG1119200.095.522391
CGGATGC11400.095.096251
CGGTAAT117000.094.337941
CGGAATA163150.094.0127941
GGCGCGG400750.093.45033
GGGAGGC485000.093.205212
CGCGGTG405450.092.616465
GCGCGGT396950.092.606384
CGGAAAT93400.090.177751
CGGATAC13900.089.562941
CGGGTTT532500.089.421381
Produced by FastQC (version 0.11.5)