FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20582351
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC874790.4250194742087529TruSeq Adapter, Index 4 (100% over 50bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC788100.3829008649206303No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT334390.1624644337277117No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA270730.13153502240827591No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG245560.11930609870563379No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT206760.10045499661336064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC393650.0114.435721
CGGGTGC128000.0113.43731
CGGTTAA1194150.0112.238891
CGGGTAC160700.0109.610431
GGGCGCG456750.0103.927842
CGGCTAA6800.0101.51381
GGCGCGG471900.097.338713
CGCGGTG472600.096.9553455
GCGCGGT469500.095.948244
CGGGCGT565950.095.578861
CGGGTGT392850.094.613091
CGGGTAT461950.094.373051
CGGAATA177100.093.747921
CGGATGC15600.092.313831
CGGGTTT1044700.092.2554861
CGGTAAT145900.091.281081
CGGTGGT872600.089.623457
GCGGTGG694600.089.456876
CGGAATT236300.087.3104251
CGGGTAA103900.087.22891
Produced by FastQC (version 0.11.5)