FastQCFastQC Report
Wed 11 May 2022
EGAF00005416006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20771737
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC775020.3731127541235478No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA406970.1959248761911438No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT316540.1523897592194625TruSeq Adapter, Index 15 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT310080.149279764133351No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG303480.1461023697729275No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT279680.13464449313988522No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA264420.12729797223987577No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA247520.11916191698363983No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA222870.10729483047084602No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT220200.10600943002503835No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT215260.10363119848860015No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT210790.10147923594449515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)