FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16185208
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT681560.42110055057679824No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC658650.40694565062123395No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA495370.30606341296324396No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA475660.29388562692552356No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA473910.29280439275170267No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG445280.2751154016679922No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA398120.24597768530376626No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT377450.2332067650906927No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT340140.21015485250483035No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT218360.13491331096887973No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA201830.12470028188701685No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT179880.11113851610680568No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT171090.10570763131372794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC314100.0116.0068361
CGGGTGC106650.0114.8921051
CGGTTAA1130600.0114.727991
CGGGTAC131050.0112.7921141
CGGCTAA7700.0109.1861651
GGGCGCG363900.0105.2366262
CGGAATA294950.099.4617161
CGGATGC12050.098.470311
CGGGCGT381300.097.923181
CGGGTGT278000.097.826051
GGCGCGG381800.097.544753
CGGGTAT322250.096.309041
GCGCGGT379800.096.2883454
CGCGGTG388600.096.052175
CGGTAAT109900.095.272171
GCTAATT8150.093.436773
CGGATAC14300.091.316091
CGGGAGG752750.090.998221
GCGGTGG566000.090.679446
CGGTGGT723150.090.496087
Produced by FastQC (version 0.11.5)