FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005416034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32329948
Sequences flagged as poor quality0
Sequence length125
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC1156300.35765600365333095No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT604110.18685770852461625TruSeq Adapter, Index 16 (97% over 40bp)
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT513910.1589578801673297No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA402070.1243645674901797No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT379060.11724732746245062No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA377810.11686068904286513No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA370330.11454704474006577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)