FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16596871
Sequences flagged as poor quality0
Sequence length125
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC671720.4047268909904765No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT261640.15764417280823595No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA255030.15366149438650212No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT242290.14598534868409835No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG213510.12864473068447663No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA195530.11781136335879214No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA180250.10860480870159202No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT167210.10074790603602331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC105250.0113.953611
CGGGCGC332100.0113.669141
CGGTTAA1024800.0113.076431
CGGGTAC124900.0109.995751
CGGCTAA7250.0103.495921
GGGCGCG395050.0102.5139542
CGGGTAT345050.096.54531
CGGAATA176150.095.809361
CGGGTGT309750.095.76261
GGCGCGG412050.094.9776843
CGCGGTG409050.094.35095
GCGCGGT406850.094.056934
CGGAAAT103200.093.1353451
CGGATAC16200.093.002841
CGGATGC11650.092.521651
CGGTAAT128650.092.162061
CGGGCGT437400.091.981731
CGGGTTT762050.091.813841
CGGTGGT723650.090.134627
CGGAATT210550.089.489471
Produced by FastQC (version 0.11.5)