FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16631337
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC489060.29405934111009835No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA383220.23042044064166337No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG374950.22544789994935463No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA374120.22494884205641436No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT231260.1390507570137025No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT226920.13644122538073758No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGT204870.12318312111648029No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA197520.11876375302839454No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA190720.11467508595370295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC277600.0113.979881
CGGTTAA860700.0113.1087341
CGGGTGC88000.0113.037721
CGGGTAC101800.0107.0176851
GGGCGCG342600.0102.924282
CGGATAC11600.098.589991
CGGCTAA6550.098.213691
CGGGCGT327600.097.3291551
GGCGCGG356200.095.759043
CGGGTAT250000.095.494261
CGCGGTG359600.094.837115
CGGGTGT219450.094.022521
GCGCGGT356800.093.914134
CGGAAAT90450.093.5124361
CGGTAAT100200.093.508391
CGGATGC11750.093.2759251
CGGAATA117900.091.544871
CGGGAGG635750.089.56971
CGGAATT168250.089.533051
GCGGTGG526300.089.302736
Produced by FastQC (version 0.11.5)