FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11155640
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CNGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC416950.37375713092211654No Hit
TNGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA385510.3455740773277015No Hit
TNGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG373680.3349695759275129No Hit
TNGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA343710.30810424144199705No Hit
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT241180.21619557461517225TruSeq Adapter, Index 14 (97% over 41bp)
CNGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT231520.20753627761383478No Hit
CNGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT231480.20750042131155183No Hit
CNGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA177770.15935437142109282No Hit
CNGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA161890.14511941941475343No Hit
CNGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT143500.12863448444015763No Hit
CNGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT117710.10551613354321222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)