FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005416065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20921852
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC737190.3523540841413083No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT387800.18535643976451033No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT381460.18232611529801473No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA319030.15248650071704933No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTAGGTGTGGGTATAA315420.15076103205395008No Hit
TGGATATTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG310590.1484524410171719No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA297370.14213368873845394No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA242190.1157593505584496No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT215390.1029497771038625No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT214760.10264865653384797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)