FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18650425
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC749540.40188896499677623No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT397840.21331417380569076No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA397460.21311042509755138No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA387060.2075341446642637No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG359010.19249427291871365No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT355420.1905693838076076No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA280100.15018424513114315No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT228870.1227157021890922No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT228380.12245297359175461No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA221110.11855493909656213No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA207400.11120390017921844No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT203960.10935943818974636No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA192070.10298424834822797No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT190680.10223895702108664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC355600.0115.016521
CGGTTAA1243550.0114.463891
CGGGTGC123000.0113.926281
CGGGTAC136450.0112.266891
CGGCTAA8950.0105.933961
GGGCGCG427550.0102.485962
CGGGCGT451550.096.1889651
CGGAATA224600.096.028451
CGGGTGT320150.095.939881
GGCGCGG443000.095.755633
CGGATGC14200.095.743131
CGGGTAT366150.095.742681
CGCGGTG444900.095.092615
GCGCGGT439550.094.463374
GCTAATT9400.092.4067153
CGGATAC17850.090.529941
CGGTAAT125550.089.907321
CGGTGGT809700.089.818897
GCGGTGG648600.089.8108146
CGGGAGG825600.088.736431
Produced by FastQC (version 0.11.5)