FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19211884
Sequences flagged as poor quality0
Sequence length125
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT974430.5072016882883532No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT648500.3375514863612543No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA601030.31284282166184224No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA513790.26743342818434673No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT479010.24933005008774778No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT437360.22765076033146983No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC413130.21503877495824983No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA332040.17283052510623112No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAATGGAA306700.15964077234694943No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAA306410.1594898241109513No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA282000.14678414672918075No Hit
TGGTGGATTTTTTGGTTTAAGTTTTGGTAATATGGTGAAATTTTGTTTTA280610.14606063621870713No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAGTGTAA250560.13041927590235294No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA233670.12162784243336053No Hit
TGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAATTTGAT231130.12030574409048066No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTTGAG227310.11831739146457475No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT221630.11536088808364656No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA212800.11076477455308391No Hit
TGGAATTGAATGGAATGGAATGGAATTAATTTGATTGTAATGGAATGGAA205920.10718365778181879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC71950.0113.288131
CGGGCGC229100.0111.599581
CGGTTAA777650.0111.406191
CGGGTAC91700.0105.197791
CGGCTAA5750.0103.624221
CGGATAC11850.099.557951
GGGCGCG289250.096.2799762
GGGAGGC459350.095.655842
CGGAATA169850.094.927351
CGGGTAT272750.094.744451
CGGGAGG983750.094.577181
CGGGTGT240500.094.1946261
CGGGTAA77250.090.93781
CGGGCGT355900.090.6398851
CGGTAAT103500.090.6136251
CGGAATG760000.090.598931
CGGGTTT565700.088.59121
GGCGCGG304350.088.563213
CGGAGAA109400.088.123211
CGCGGTG307700.088.101095
Produced by FastQC (version 0.11.5)