FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20689518
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC618660.2990209825091141No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT448890.2169649384775421No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT402090.1943447885059478No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA345760.16711844132859935No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA339730.16420392200533623No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG312940.15125533615621206No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA304710.14727747644966888No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA253520.12253547907689294No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT246380.11908445619661125No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATTTTGTTAGTT213550.10321651765884542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1443700.0113.485851
CGGGTGC126900.0112.986321
CGGGCGC330200.0109.52811
CGGGTAC137250.0108.1505361
CGGCTAA9950.0107.028751
CGGAATA272200.097.3273851
GGGCGCG398000.096.653772
CGGATGC15600.096.1050951
CGGGTGT358600.095.130071
CGGGTAT385050.093.107031
CGGGCGT448900.092.957961
CGGATGA90000.092.876171
CGGATAA115300.092.620181
CGGTAAT143350.091.263691
CGGAAAT122100.090.677831
CGGGTTT940200.089.683391
GGCGCGG420250.088.691873
CGCGGTG417200.088.143225
CGGAATT267050.088.1098251
CGGGAGG869600.087.783211
Produced by FastQC (version 0.11.5)