FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20125370
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC808850.40190565440535997No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT714060.3548058992207348No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA527550.2621318266446778No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA502140.24950597181567347No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA463090.23010260184036369No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA405050.2012633804993399No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG402620.20005594928192622No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT370080.18388730244462587No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT332390.165159696442848No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT306160.15212639568862585No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA256330.12736660245252635No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA222590.11060169328563899No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT220120.1093743866572391No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA214790.10672598814332357No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGT210960.1048229175413918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC137750.0114.8575741
CGGGCGC409650.0113.062521
CGGTTAA1186450.0113.013651
CGGGTAC164450.0110.1810761
CGGCTAA8350.0102.653151
GGGCGCG484700.0100.854112
CGGGTGT361150.098.743381
CGGAATA330800.098.30191
CGGGTAT415900.096.993991
CGGGCGT507600.096.944371
CGGATGC15450.095.93281
CGGATAC16650.095.8113251
CGGAAAT128650.095.498411
CGGTAAT151750.094.140991
CGGGAGG1196950.093.985111
GGCGCGG503900.093.893293
GGGAGGC497800.093.76632
CGCGGTG509450.092.292465
GCGCGGT506150.091.382644
CGGGTTT707050.088.640711
Produced by FastQC (version 0.11.5)