FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17644137
Sequences flagged as poor quality0
Sequence length125
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC497720.28208803864989257No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT335790.1903125100422877No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT305880.17336070333165063No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA291190.165034991510211No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA272450.15441389964269717No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG254590.14429155701976243No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA228500.12950477543900277No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA213340.12091268618011752No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT200740.11377150381455324TruSeq Adapter, Index 18 (97% over 40bp)
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT188090.1066019834237288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA1183600.0113.0124741
CGGGTGC107150.0112.331121
CGGGCGC274400.0108.1963961
CGGGTAC119300.0106.0276641
CGGCTAA8300.0103.224031
CGGAATA221250.097.4274751
CGGGTGT306450.095.366341
GGGCGCG337350.094.992362
CGGATGC12600.093.9677661
CGGGTAT327400.093.3164441
CGGAAAT112750.092.820881
CGGGCGT389800.092.527431
CGGATGA78500.092.391171
CGGATAA98100.092.308571
CGGTAAT127750.092.307951
CGGGAGG774300.090.594311
CGGAATT224900.089.735191
CGGGTAA82950.089.6581951
CGGGTTT756750.089.581881
GGGAGGC314550.089.192732
Produced by FastQC (version 0.11.5)