FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19110619
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT1026610.537193483895001No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA751870.39343047967206085No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC638310.33400801931114843No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT565500.29590878244184554No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA537920.2814770154750089No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT518070.27109012010547645No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA391060.20462968781911253No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA339690.177749344487481No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT322380.16869155310981818No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA311640.16307164095521973No Hit
CGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA298250.15606506518705648No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT295830.15479875350976333No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT290660.15209345129009164No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT282380.14776078158431186No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA278400.14567816981752396No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT260580.1363535111029109No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA248160.1298545065442412No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA247120.12931030648457803No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAA235030.12298398079099375No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGAT219630.11492563375367382No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG203650.10656379052923404No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATTAATTCGAGTGTAATGGAA195100.10208983811565706No Hit
CGGAACGGAATGGAATGGAATGGAATGGAATTAATTCGATTGTAATGGAA193500.10125260725463682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC132450.0113.420231
CGGTTAA1009750.0112.3176961
CGGGCGC338950.0109.484831
CGGGTAC143300.0107.5738451
CGGCTAA7050.0107.2181
CGGATGC12450.099.436791
CGGAATA409300.099.086271
GGGCGCG410650.098.2742542
CGGGTGT327600.098.253021
CGGGTAT369050.096.668431
CGGTAAT136600.096.336571
CGGGAGG1119350.095.003331
GGGAGGC477500.094.507272
CGGGCGT439050.093.795591
CGGGTAA96300.092.275541
CGGATGA75900.091.356041
GGCGCGG433800.090.065633
CGGGAAG177300.089.5297551
CGGGTTT604300.089.145021
GCGCGGT430450.088.91364
Produced by FastQC (version 0.11.5)