FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21731510
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC735890.3386281026951187No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT551270.2536731225763879No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA447300.20583015170137742No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA335580.15442093071305216No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT325150.14962144830248794No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT258790.11908514410641506TruSeq Adapter, Index 13 (97% over 40bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA237940.1094907808983361No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT233480.10743846147828659No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT229550.10563002754985731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC127450.0115.8886261
CGGGCGC374300.0114.397751
CGGTTAA938650.0113.337291
CGGGTAC154800.0110.5261461
GGGCGCG447300.0100.640512
CGGCTAA6500.099.768981
CGGGTAT487100.097.1882251
CGGGTGT422200.095.4184951
CGGAATA287100.094.932481
GGGAGGC583350.094.471762
CGGGTTT974150.094.356181
CGCGGTG457900.093.848675
GGCGCGG466450.093.3800053
GCGCGGT457700.093.2136464
CGGATGC13450.091.9248661
CGGGAGG1438000.091.855081
AAGCAGA31900.091.385971
CGGGTCT4250.091.26751
CGGGCGT652850.090.8947451
CGGTAAT142900.090.423251
Produced by FastQC (version 0.11.5)