FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20192930
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC601310.2977824416763689No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT477140.23629062250995772No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA352140.1743877683922046No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT292450.14482791749389515No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT288970.14310454203525688No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA286240.14175258370132518No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA279860.13859306202715505No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT279500.13841478180729594No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAG242060.11987363894194651No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAA234020.11589204736509263No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA229020.11341593320038251No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA204810.10142658841485609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC130950.0113.2010651
CGGTTAA1110050.0112.606241
CGGGCGC343600.0110.115811
CGGGTAC140700.0107.133031
CGGCTAA6850.099.03681
GGGCGCG424900.098.080482
CGGGTGT353950.096.8081741
CGGGTAT381350.095.4546361
CGGAATA258700.095.278731
CGGATAC12950.095.122421
CGGTAAT142250.093.624631
CGGGCGT459500.093.362361
CGGATGC14850.092.168751
CGGAAAT118650.091.382481
CGGGAGG937200.090.749251
GGCGCGG447000.090.300843
CGGGTTT769250.089.737261
GGGAGGC386800.089.158972
CGGGTAA92600.088.8135761
CGCGGTG450950.088.6654365
Produced by FastQC (version 0.11.5)