FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005416132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005416132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22585298
Sequences flagged as poor quality0
Sequence length125
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT831100.3679827470064818No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA592610.2623875053585744No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGAAATGTAATGGATTTAAT560400.24812601542826665No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC484140.21436068720456997No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA435570.19285554700230212No Hit
CGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT429860.1903273536616608No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA428390.18967648777536608No Hit
TGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTTGAT324340.1436066949393362No Hit
CGGAATGGAATGGAATGGAATGGAATGTAAAGTAATGGAATTAATTCGAT311500.13792158066721102No Hit
TGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATTAAT304780.13494619375843525No Hit
TGGAATAGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAA288120.12756971371376194No Hit
CGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA231270.1023984717846096No Hit
TGGAATGGAATGGAATGGAATGGAATTAATTTTATTGTAATGGAATGGAA230480.10204868671646483No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA229000.10139339317107969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGC121000.0111.854181
CGGTTAA800650.0108.1899261
CGGGCGC280750.0104.853161
CGGGTAC118550.0104.074641
CGGCTAA7250.0103.4379041
GGGAGGC656500.096.827212
CGGAATA326550.094.466681
CGGGAGG1554900.094.139931
CGGGTGT410550.093.8977051
CGGGTAT434050.093.105891
GGGCGCG338150.093.103612
CGGGTTT848000.092.6878051
CGGTAAT141000.091.9360661
CGGGTAA120550.090.7455751
GCGGAGT423400.087.867837
CGGGAAG218450.086.150341
CGGAGTG141150.085.850741
CGGAATT266850.085.267641
GCGCGGT350350.085.05294
GGCGCGG358900.084.649843
Produced by FastQC (version 0.11.5)